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免疫球蛋白样结构域受体1抗体

作者:上海雅吉生物科技有限公司 2020-11-09T00:00 (访问量:1190)

  

中文名称 免疫球蛋白样结构域受体1抗体
别    名 ILDR1alpha; ILDR1beta; Immunoglobulin like domain containing receptor 1 alpha; Immunoglobulin like domain containing receptor 1 alpha; Immunoglobulin like domain containing receptor 1; Immunoglobulin like domain containing receptor 1 beta; MGC50831; ILDR1_HUMAN.  
研究领域 细胞生物  免疫学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Pig, Cow, Horse, Rabbit, Sheep, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=3ug/Test 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 60kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ILDR1:101-200/546 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 ILDR1 is a putative membrane receptor. It belongs to the immunoglobulin superfamily, LISCH7 family. It is mainly expressed in prostate and to a lower extent in testis, pancreas, kidney, heart and liver.

Function:
Putative membrane receptor.

Subunit:
Homooligomer.

Subcellular Location:
Cell membrane; Single pass type I membrane protein. Isoform 5: Cytoplasm (cytosol).

Tissue Specificity:
Mainly expressed in prostate and to a lower extent in testis, pancreas, kidney, heart and liver.

DISEASE:
Defects in ILDR1 are the cause of deafness autosomal recessive type 42 (DFNB42) [MIM:609646]; also called non-syndromic sensorineural deafness autosomal recessive type 42. DFNB42 is a prelingual, non-progressive form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Similarity:
Belongs to the immunoglobulin superfamily. LISCH7 family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
Q86SU0

Gene ID:
286676

Database links:

Entrez Gene: 286676 Human

Entrez Gene: 106347 Mouse

Omim: 609739 Human

SwissProt: Q86SU0 Human

SwissProt: Q8CBR1 Mouse

Unigene: 98484 Human

Unigene: 17807 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Sample:
Prostate(Rat) Cell Lysate at 40 ug
Primary: Anti-ILDR1 (bs-11013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56 kD
Sample:
DU145(Human) Cell Lysate at 30 ug
Primary: Anti-ILDR1 (bs-11013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56 kD
Sample:
Pancreas(Mouse) Cell Lysate at 40 ug
Primary: Anti-ILDR1 (bs-11013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56kD
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-ILDR1 antibody (bs-11013R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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