Mouse Stem Cell Factor Retroviral Vector Set
产品名称： Mouse Stem Cell Factor Retroviral Vector Set
英文名称： Mouse Stem Cell Factor Retroviral Vector Set
产品编号： RTV-705-C
产品价格： null
产品产地： USA
品牌商标： cellbiolabs
更新时间： 2024-12-10T13:16:28
使用范围：
翌圣生物科技（上海）股份有限公司
- 联系人 : 李自转
- 地址 : 上海市浦东新区天雄路166弄一号楼三层南单元
- 邮编 : 200030
- 所在区域 : 上海
- 电话 : 139****5640 点击查看
- 传真 : 点击查看
- 邮箱 : lizizhuan@yeasen.com
- 二维码 : 点击查看
Kit Components 1. pMXs-mOct3/4 (Part No 370501): One vial containing 10 μg at 0.25 μg/μL in TE. 2. pMXs-mSox2 (Part No 370502): One vial containing 10 μg at 0.25 μg/μL in TE. 3. pMXs-mc-Myc (Part No 370503): One vial containing 10 μg at 0.25 μg/μL in TE. 4. pMXs-mKlf4 (Part No 370504): One vial containing 10 μg at 0.25 μg/μL in TE.
Background
Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. Cell
Biolabs’ pMXs retroviral vector is based on Moloney murine leukemia virus (MMLV). The vector
provides the viral package signal, transcription and processing elements, and MCS for cloning of a target
gene. The viral env gene, produced by the package cell line, encodes the envelop protein, which
determines the viral infectivity range. Transfection into a package cell line produces high-titer,
replication-incompetent viruses.
Induced pluripotent stem (iPS) cells can be generated from various somatic cells by the retrovirusmediated
transfection of four transcription factors, namely Oct3/4, Sox2, c-Myc, and Klf4. iPS cells are
indistinguishable from ES cells in morphology, proliferation, gene expression, and teratoma formation.
Furthermore, when transplanted into blastocysts, iPS cells can give rise to adult chimeras, which are
competent for germline transmission.