Product: Anti-Ku (p70) mAb, clone N3H10
Cat. No.: MC-351 (100 μg)
Background:
The Ku autoantigen is a heterodimer of 70 kDa
(p70) and ~80 kDa (p80) proteins. The p70/p80
dimer is important for the function of a 460 kDa
DNA-dependent protein kinase that
phosphorylates certain transcription factors,
including Sp1, Oct-1, p53 and SV40 large T
antigen in vitro. Ku protein plays a role in cell
signaling, proliferation, DNA repair, replication,
transcriptional activation and apoptosis.
Specificity:
Reacts with the p70 Ku antigen subunit.
Recognizes an epitope between aa 506-541 of
p70.
Species Reactivity:
Human, monkey and Xenopus. Mouse and
hamster (Western blots only). Does not react
with bovine, rabbit or sea urchin. Other species
not tested.
Cellular Localization:
Nuclear
Positive Control:
BT474, T47D, HEP-G-2 or HeLa cells. Tonsil.
Ig Isotype:
Mouse IgG2b
Immunogen:
Human placental extract designated as PSE1-
PL.
Format:
100 μg of monoclonal antibody at 200 μg/mL in
10 mM PBS, pH 7.4, with protein stabilizer and
0.09 sodium azide. Purified from ascites fluid
by Protein A chromatography.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Immunofluorescence

Immunohistochemistry: (Formalin/paraffin)
Use at a 1:400 dilution for 30 minutes at room
temperature (RT). Staining of formalin-fixed
tissues is improved by boiling tissue sections
in 10 mM citrate buffer, pH 6.0, for 10-20 min.
followed by cooling to RT for 20 min.

Immunoprecipitation: (Native and denatured)
Use at 2 μg/mg protein lysate. Use Protein A.

Western blot: Use at 0.25-0.5 μg/mL for 2 hrs.
at RT.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.